Rheomuco: 3 steps in 10 minutes. Fast and easy!

With Rheomuco you can analyse the physical properties of sputum in your laboratory. The protocol includes three simple steps:

Step 1: collect mucus sample with minimum volume 76 µl

The donor’s sputum is obtained spontaneously or induced by inhaling hypertonic saline which improves the mucociliary clearance of the lower airways.

Step 2: prepare the sample for measurement according to a homogenisation protocol (3 minutes)

At this stage, the sample can be mixed with a drug to test and/or be incubated under the conditions stipulated in your research protocol.

Step 3: deposit the sample and measure the viscoelasticity using Rheomuco (7 minutes)

Let the software guide you: only one measurement is possible per sample as the analysis modifies the initial physical properties.

Rheomuco can be used in a research laboratory as well as in clinical trials as a biophysical marker for all research stages.

The results are displayed at the end of each measurement as well as exported into a PDF report

Here are the results displayed by Rheomuco:

  • Viscoelastic modulus G*p: General consistency of the mucus at rest, molecular network strenght
  • Elastic modulus G’p: Elastic characteristic of mucus at rest, energy storage capacity
  • Viscous modulus G”p: Viscous characteristic of mucus at rest, energy dissipation capacity
  • Damping factor Tan(δ)p: Dissipation over storage capacity, molecular network morphology
  • Crossover Strain γc: Stretchability of the mucus before flow
  • Crossover yield stress τc: Gel force, firmness of the mucus before flow
  • Elastic force (EF) G*p. τc: Maximum storable elastic energy